README for Figure S7.csv 
*** This file contains the raw data obtained on DNA and DNA-sRok complexes using microscale thermophoresis experiments represented in Figure S7 of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es


Legend Figure S7: MgCl2 does affect the DNA binding affinity of sRok Kd-values (mM) obtained from fitting protein 
titration data to the McGhee-von Hippel equation as a function of the MgCl2 concentration. The DNA used was 78 bp 
in length with or without a specific Rok binding site and the sRok concentration was varied between 0.125 and 16 
M. The Rok data was reproduced from figure S2 for comparison. The final measurement buffer consisted of 10 mM 
Tris HCl pH 8, 150 mM KCl, 5% glycerol, 0.05% Tween20 and 0.08 mg/ml acetylated BSA. MgCl2 was added accordingly. 
Each data point was measured at least in triplicate and error bars represent the standard deviation. Some error 
bars are hidden behind the data points due to their small size.  

*** The data were obtained using microscale thermophoresis experiments as described in the associated article. 

***The data are labeled by type of DNA used (Aspecific/Specific) and mM MgCl2 as represented in figure S7.
Column A: Amount of Rok in micromolar
Column B: Fnorm values replicate 1
Column C: Fnorm values replicate 2
Column D: Fnorm values replicate 3
Column E: Fnorm values replicate 4










